![JAIST 高木研究室-北陸先端科学技術大学院大学 マテリアルサイエンス研究科 機能科学専攻[バイオ系]](../img/top.jpg) |
論文リスト（2001年～）
Kazuki Uchida, Hiroki Obayashi, Kosuke Minamihata, Rie Wakabayashi, Masahiro Goto, Naofumi Shimokawa, Masahiro Takagi, and Noriho Kamiya
Artificial Palmitoylation of Proteins Controls the Lipid Domain-Selective Anchoring on Biomembranes and the Raft-Dependent Cellular Internalization
Langmuir, 38, 9640-9648 (2022).doi
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa and Manish Biyani
Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Scientific Reports, 11, 15997 (2021).doi
Jingyu Guo, Hiroaki Ito, Yuji Higuchi, Klemen Bohinc, Naofumi Shimokawa, and Masahiro Takagi
Three-Phase Coexistence in Binary Charged Lipid Membranes in a Hypotonic Solution
Langmuir, 37, 9683-9693 (2021).doi
Wanjae Choi, Hyunil Ryu, Ahmed Fuwad, Seulmini Goh, Chaoge Zhou, Jiwook Shim, Masahiro Takagi, Soonjo Kwon, Sun Min Kim, and Tae-Joon Jeon
Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane
Membranes, 11, 579 (2021).doi
下川直史, 高木昌宏
界面活性剤刺激性評価法
月刊PHARMSTAGE, 21(2), 49-55 (2021).
Nichaporn Wongsirojkul, Naofumi Shimokawa, Pakorn Opaprakasit, Masahiro Takagi, and Tsutomu Hamada
Osmotic-Tension-Induced Membrane Lateral Organization
Langmuir, 36, 2937-2945 (2020).doi
下川直史, 高木昌宏
第16章/第1節 界面活性剤刺激性評価法
界面活性剤の選び方、使い方 事例集(情報技術協会), (2019).
Naofumi Shimokawa, Hiroaki Ito, and Yuji Higuchi
Coarse-grained molecular dynamics simulation for uptake of nanoparticles into a charged lipid vesicle dominated by electrostatic interactions
Phys. Rev. E, 100, 012407 (2019).doi
Neha Sharma, KeangOk Baek, Naofumi Shimokawa, and Masahiro Takagi
Effect of temperature on raft-dependent endocytic cluster formation during activation of Jurkat T cells by concanavalin A
J. Biosci. Bioeng., 127, 479-484 (2019). doi
Neha Sharma, Huong T. T. Phan, Tsuyoshi Yoda, Naofumi Shimokawa, Mun'delanji C. Vestergaard, and Masahiro Takagi
Effects of capsaicin on biomimetic membranes
Biomimetics, 4, 17 (2019).doi
下川直史, 高木昌宏
生体模倣膜を用いた界面活性剤刺激性評価法
繊維機械学会誌 月刊せんい, 71, 527-531 (2018).
下川直史, 高木昌宏
細胞模倣膜の相分離構造と脂肪酸
生体の科学, 69(3), 218-222 (2018).
Miyuki Miyake, Yasushi Kakizawa, Norio Tobori, Masatoshi Kurioka, Nobuhito Tabuchi, Ryo Kon, Naofumi Shimokawa, Yoshio Tsujino, and Masahiro Takagi
Membrane permeation of giant unilamellar vesicles and corneal epithelial cells with lipophilic vitamin nanoemulsions
Colloids Surf. B-Biointerfaces, 169, 444-452 (2018).doi
Huong T. T. Phan, Naofumi Shimokawa, Neha Sharma, Masahiro Takagi, and Mun'delanji C. Vestergaard
Strikingly different effects of cholesterol and 7-ketocholesterol on lipid bilayer-mediated aggregation of amyloid beta (1-42)
Biochem. Biophys. Rep., 14, 98-103 (2018).doi
Shoko Ichikawa, Naofumi Shimokawa, Masahiro Takagi, Yukiya Kitayama, and Toshifumi Takeuchi
Size-dependent uptake of electrically neutral amphipathic polymeric nanoparticles by cell-sized liposomes and an insight into their internalization mechanism in living cells
Chem. Commun., 54, 4557-4560 (2018).doi
Kazushi Kinbara, Kaori Umetsu, Hiroki Sonobe, Takahiro Muraoka, Naofumi Shimokawa, and Masahiro Takagi
Localization of transmembrane multiblock amphiphilic molecules in phase-separated vesicles
Faraday Discuss., 209, 315-328 (2018).doi
Pooja Gusain, Shinya Ohki, Kunihide Hoshino, Yoshio Tsujino, Naofumi Shimokawa, and Masahiro Takagi
Chirality-dependent interaction of D- and L-menthol with biomembrane models
Membranes, 7, 69 (2017).doi
Satomi Yabuuchi, Satoshi Endo, KeangOk Baek, Kunihide Hoshino, Yoshio Tsujino, Mun'delanji C. Vestergaard, and Masahiro Takagi
Raft-dependent endocytic movement and intracellular cluster formation during T cell activation triggered by concanavalin A
J. Biosci. Bioeng., 124, 685 (2017).doi
Ko Sugahara, Naofumi Shimokawa, and Masahiro Takagi
Thermal stability of phase-separated domains in multicomponent lipid membranes with local anesthetics
Membranes, 7, 33 (2017).doi
Naofumi Shimokawa, Rieko Mukai, Mariko Nagata, and Masahiro Takagi
Formation of modulated phase and domain rigidification in fatty acids-containing lipid membranes
Phys. Chem. Chem. Phys., 19, 13252-13263 (2017).doi
Neha Sharma, KeangOK Baek, Huong T. T. Phan, Naofumi Shimokawa, and Masahiro Takagi
Glycosyl chains and 25-hydroxycholesterol contribute to the intracellular transport of amyloid beta (Aβ-42) in Jurkat T cells
FEBS Open Bio, 7, 865-876 (2017).doi
下川直史, 高木昌宏
第2章/第7節 膜弱秩序構造のダイナミクス
人工細胞の創製とその応用(シーエムシー出版),p. 117-126 (2017).
下川直史, 高木昌宏
分子間の弱い相互作用と生体秩序-生体模倣膜の相分離構造
月刊「化学」, 72(2), 68-69 (2017).
下川直史, 高木昌宏
生物、物理、数学の境界領域としての膜研究
生物工学会誌, 94, 746-749 (2016).
Hiroaki Ito, Yuji Higuchi, and Naofumi Shimokawa
Coarse-grained molecular dynamics simulation of charged lipid membranes: Phase separation and morphological dynamics
Phys. Rev. E, 94, 042611 (2016).doi
下川直史, 高木昌宏
2次元・3次元 膜ダイナミクスと細胞信号伝達
膜(MEMBRANE), 41, 233-239 (2016).
下川直史,
第8章/第9節 脂質膜の相分離
材料表面の親水・親油の評価と制御設計 (テクノシステム),p.531-539 (2016).
Naofumi Shimokawa, Hiroki Himeno, Tsutomu Hamada, Masahiro Takagi, Shigeyuki Komura, and David Andelman
Phase diagrams and ordering in charged membranes: Binary mixtures of charged and neutral lipids
J. Phys. Chem. B, 120, 6358 (2016).doi
Ryuichi Okamoto, Naofumi Shimokawa, and Shigeyuki Komura
Nano-domain formation in charged membranes: Beyond the Debye-Hückel approximation
EPL, 114, 28002 (2016).doi
Hiroki Himeno, Hiroaki Ito, Yuji Higuchi, Tsutomu Hamada, Naofumi Shimokawa, and Masahiro Takagi
Coupling between pore formation and phase separation in charged lipid membranes
Phys. Rev. E, 92, 062713 (2015).doi
Ko Sugahara, Naofumi Shimokawa, and Masahiro Takagi
Destabilization of Phase-separated Structures in Local Anesthetic-containing Model Biomembranes
Chem. Lett., 44, 1604-1606 (2015).doi
Naofumi Shimokawa, Mariko Nagata, and Masahiro Takagi
Physical properties of the hybrid lipid POPC on micrometer-sized domains in mixed lipid membranes
Phys. Chem. Chem. Phys., 17, 20882-20888(2015).doi
Tsutomu Hamada, Rie Fujimoto, Shunsuke F. Shimobayashi, Masatoshi Ichikawa, Masahiro Takagi
Molecular behavior of DNA in a cell-sized compartment coated by lipids
Phys. Rev. E, 91, 062717(2015).doi
Huong T. T. Phan, Mun'delanji C. Vestergaard, KeangOk Baek, Naofumi Shimokawa, and Masahiro Takagi
Localization of amyloid beta (Aβ1-42) protofibrils in membrane lateral compartments: effect of cholesterol and 7-ketocholesterol
FEBS Lett., 588, 3483-3490(2014).doi
Hiroki Himeno, Naofumi Shimokawa, Shigeyuki Komura, David Andelman, Tsutomu Hamada, and Masahiro Takagi
Charge-induced phase separation in lipid membranes
Soft Matter, 10, 7959-7967(2014).doi
Tatsuya Shima, Takahiro Muraoka, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Akihiko Ishijima, Yuki Omata, Takashi Yamashita, and Kazushi Kinbara
Micrometer-Size Vesicle Formation Triggered by UV Light
Langmuir, 30, 7289-7295(2014).doi
Hiroaki Ito, Toru Yamanaka, Shou Kato, Tsutomu Hamada, Masahiro Takagi, Masatoshi Ichikawa and Kenichi Yoshikawa
Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface
Soft Matter, 9, 9539-9547(2013).doi
Huong Thi Thanh Phan, Takahiro Hata, Masamune Morita, Tsuyoshi Yoda, Tsutomu Hamada, Mun'delanji C Vestergaard and Masahiro Takagi
The effect of oxysterols on the interaction of Alzheimer's amyloid beta with model membranes
Biochim. Biophys. Acta, Biomembr. , 1828, 2487-2495(2013).doi
Shruti Dhingra, Masamune Morita, Tsuyoshi Yoda, Mun'delanji C. Vestergaard, Tsutomu Hamada and Masahiro Takagi
Dynamic morphological changes induced by GM1 and protein interactions on the surface of cell-sized liposomes
Materials, 6, 2522-2533(2013).doi(Open Access)
Norihiro Takei,Nobuteru Takahashi,Tomohiro Takayanagi,Atsuo Ikeda,Masahiro Takagi,Tsutomu Hamada,Eiichi Saitoh,Akihito Ochiai,Takaaki Tanaka,Masayuki Taniguchi
Antimicrobial avtivity and mechanism of action of a novel cationic α helical dodecapeptide,a partial sequence of cyanate lyase from rice
Peptides, 42, 55-62(2013).doi
Mun'delanji C. Vestergaard,Masamune Morita,Tsutomu Hamada,Masahiro Takagi
Membrane fusion and vesicular transformation induced by Alzheimer's Amyloid beta
Biochim.Biophys.Acta,Biomembr. .,1828,1314-1321(2013).doi
Takahiro Muraoka, Tatsuya Shima, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Kazuhito V. Tabata, Hiroyuki Noji and Kazushi Kinbara
Ion permeation by a folded multiblock amphiphilic oligomer achieved by hierarchical construction of self-assembled nanopores
J. Am. Chem. Soc. , 134, 19788-19794(2012).doi
Natsuko Miura, Aya Kirino, Satoshi Endo, Hironobu Morisaka, Kouichi Kuroda, Masahiro Takagi, Mitsuyoshi Ueda
Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion
Eukaryotic Cell, 11, 1075-1082(2012).doi
Tsutomu Hamada, Masamune Morita, Makiyo Miyakawa, Ryoko Sugimoto, Ai Hatanaka, Mun'delanji C. Vestergaard, Masahiro Takagi
Size-denpendent partitioning of nano/micro-particles mediated by membrane lateral heterogeneity
J. Am. Chem. Soc. ., 134, 13990-13996(2012).doi
Yoshifumi Yajima, Mun'delanji C. Vestergaard, Masahiro Takagi
Damage in brain development by morpholino knockdown of zebrafish dax1
Journal of Bioscience and Bioengineering, 113, 683-688(2012).doi
Tsuyoshi Yoda, Mun'delanji C. Vestergaard, Tsutomu Hamada, Phuc Thi Minh Le, Masahiro Takagi
Thermo-induced vesicular dynamics of membranes containing cholesterol derivatives
Lipids, 47, 813-820(2012).doi
Masamune Morita, Tsutomu Hamada, Yuiko Tendo, Takahiro Hata, Mun'delanji C. Vestergaard, Masahiro Takagi
Selective localization of Alzhimer's amyloid beta in membrane lateral compartments
Soft Matter, 8, 2816-2819(2012).doi
Tsutomu Hamada, Hideyuki Hagihara, Masamune Morita, Mun'delanji C. Vestergaard, Yoshio Thujio, Masahiro Takagi
Physicochemical profiling of surgfactant-induced membrane dynamics in a cell-sized liposome
J. Phys. Chem. Lett. , 3, 430-435(2012).doi
Tsutomu Hamada, Yuko Kishimoto, Takeshi Nagasaki, Masahiro Takagi
Lateral phase separation in tense membranes
Soft matter, 7, 9061-9068(2011).doi
Tsutomu Hamada, Ryoko Sugimoto, Takeshi Nagasaki, and Masahiro Takagi
Photochemical control of membrane raft organization
Soft Matter, 7, 220-224(2011). doi
Movie of photo-induced reversible phase separation 1 (in real speed)
Movie of photo-induced reversible phase separation 2 (in real speed)
Mun'delanji C. Vestergaard, Tsuyoshi Yoda, Tsutomu Hamada, Yoko Akazawa-Ogawa, Yasukazu Yoshida, Masahiro Takagi
The effect of oxycholesterols on thermo-induced membrane dynamics
Biochim.Biophys.Acta, Biomembr. , 1808, 2245-2251(2011).
doi
Takahiro Muraoka, Tatsuya Shima, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, and Kazushi Kinbara
Mimicking multipass transmembrane proteins: synthesis, assembly and folding of alternating amphiphilic multiblock molecules in liposomal membranes
Chem. Comm. , 47, 194-196 (2011).doi
Tsutomu Hamada, Ryoko Sugimoto, Mun’delanji Vestergaard, Takeshi Nagasaki, and Masahiro Takagi
Membrane disc and sphere: controllable mesoscopic structures for the capture and release of a targeted object
J. Am. Chem. Soc. , 132, 10528-10532 (2010).doi
Movie of membrane closing dynamics (in real speed)
Movie of membrane opening dynamics (in real speed)
Movie of membrane edge fluctuation (in real speed)
Movie of the capture and release of a colloidal particle (in real speed)
Tsuyoshi Yoda, Mun'delanji C. Vestergaard, Yoko Akazawa-Ogawa, Yasukazu Yoshida, Tsutomu Hamada, Masahiro Takagi
Dynamic response of a cholesterol-containing model membrane to oxidative stress
Chem. Lett. , 39, 1273-1274 (2010).
PDF(Open Access)
Masamune Morita, Mun’delanji Vestergaard, Tsutomu Hamada,
Masahiro Takagi
Real-time observation of model-membrane dynamics induced by
Alzheimer's amyloid beta
Biophysical Chemistry, 147, 81-86 (2010).
doi
Mun’delanji Vestergaard, Tsutomu Hamada, Masamune Morita,
Masahiro Takagi
Cholesterol, lipids, amyloid Beta, and Alzheimer's.
Current Alzheimer Research, 7, 262-270 (2010).
Abstract
Tsutomu Hamada, Masamune Morita, Yuko Kishimoto, Yuuki Komatsu,
Mun'delanji Vestergaard, Masahiro Takagi
Biomimetic microdroplet membrane interface: detection of the
lateral localization of amyloid beta peptides
J. Phys. Chem. Lett., 1, 170-173 (2010).
doi
濵田勉，
脂質ベシクルの形状安定性と変形・_イナ・~クス
月刊機能材料、特集「ソフトマター研究の最前線」 (シーエムシー出版), Vol.30, No.7,p.50-55 (2010).
濵田勉，小松佑規，高木昌宏,
第3章 細胞内生体分子群の実測定量解析/第3節 細胞模倣非対称２分子膜リポソームの構築
シングルセル解析の最前線 (シーエムシー出版),p.149-157 (2010).
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Yasuhisa Hori, Tomoaki Yoshikawa, Naoki Tsuji, Takeshi Bamba, Yoshikazu Aso,
Motonori Kudou, Yoshiki Uchida, Masahiro Takagi, Kazuo Harada and Kazumasa Hirata
Phytochelatins inhibit the metal-induced aggregation of a-crystalin
J. Biosci. Bioeng., 107,173-176(2009).
PDF(Open Access)
Minhaz Uddin Ahmed, Masato Saito, M. Mosharraf Hossain, S. Ramachandara Rao,
Satoshi Furui, Akihiro Hino, Yuzuru Takamura, Masahiro Takagi and Eiichi Tamiya
Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification
Analyst , 134,966-972(2009).
Abstract
Sachiko Matsumoto, Mun'delanji Vestergaard, Takafumi Konishi,
Shingo Nishikori,
Kentaro Shiraki, Naoki Tsuji, Kazumasa Hirata, and Masahiro Takagi.
Role of C-terminal Cys-rich region of phytochelatin synthase in tolerance to cadmium ion toxicity
J.Plant Biochem. Biotech., 18,175-180 (2009).
Tsutomu Hamada, Yuichi Hirabayashi, Takao Ohta, Masahiro Takagi
Rhythmic pore dynamics in a shrinking lipid vesicle
Physical Review E, 80,051921(2009).
doi
PDF (JAIST Repository)
Movie of rhythmic-pore shrinkage
Movie of continuous-pore shrinkage
Masae Ohno, Tsutomu Hamada, Kingo Takiguchi, Michio Homma
Dynamic behaviors of giant liposomes in desired osmotic pressures
Langmuir, 25,11680-11685 (2009).
doi
Ken-ichi Ishii, Tsutomu Hamada, Masaomi Hatakeyama, Ryoko Sugimoto,
Takeshi Nagasaki, Masahiro Takagi
Reversible Control of Exo- and Endo-Budding Transitions in a
Photosensitive Lipid Membrane
ChemBioChem, 10,251-256 (2009).
doi
Movie files are available
Shin-ichiro M. Nomura, Tsutomu Hamada
Artificial-cellular computing
International Journal of Unconventional Computing, 5,87-102(2009)
Abstract
Shinji Kakimoto, Tsutomu Hamada, Yuuki Komatsu, Masahiro Takagi,
Toshizumi Tanabe, Hideki Azuma, Seiji Shinkai, and Takeshi Nagasaki
The conjugation of diphtheria toxin T domain to poly(ethylenimine)based
vectors for enhanced endosomal escape during gene transfection
Biomaterials, 30,402-408(2009).
doi
山田彩子，濵田勉，吉川研一
細胞サイズリポソームの新しい作成法とその応用
生物物理, Vol.49, p.256-259.(2009)
濵田勉、高木昌宏、小松佑規
マイクロ液滴の作製方法,
特願2009-234643
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Tsutomu Hamada, Yoko Miura, Yuuki Komatsu, Yuko Kishimoto, Mun’delanji
Vestergaard, Masahiro Takagi
Construction of asymmetric cell-sized lipid vesicles from lipid-coated
water-in-oil micro-droplets
J. Phys. Chem. B, 112,14678-14681(2008)
doi
Mun'delanji Vestergaard, Tsutomu Hamada, Masato Saito, Yoshifumi Yajima, Motonori Kudou, Eiichi Tamiya, Masahiro Takagi
Detection of Alzheimer’s amyloid beta aggregation by capturing molecular trails of individual assemblies
Biochem. Biophys. Res. Commun., 377, 725-728 (2008)
doi
IZUMIKAWA, Naoshige; NISHIKORI, Shingo;
VESTERGAARD, Mun'delanji; HAMADA, Tsutomu; HAGIHARA, Yoshihisa; YUMOTO,
Noboru; SHIRAKI, Kentaro; TAKAGI, Masahiro
Effect of Phospholipids
on conformational structure of bovine pancreatic trypsin inhibitor (BPTI)
and its thermolabile mutants
Biopolymers, 89, 873-880 (2008).
doi
Vestergaard M, Matsumoto S, Nishikori S, Shiraki K, Hirata K, Takagi M.
Chelation of Cadmium Ions by Phytochelatin Synthase: Role of the Cystein-rich C-Terminal
Anal Sci., 24, 277-281 (2008).
PDF(Open Access)
Mun'delanji Vestergaard, Tsutomu Hamada, Masahiro Takagi
Using Model Membranes for the Study of Amyloid beta:Lipid Interactions and Neurotoxicity
Biotechnology and Bioengineering, 99, 753-763 (2008).
doi
濵田勉
生命化学研究法 巨大リポソームの顕微鏡直接観察
生命化学研究レター, No.28, 27-31, 2008.
高木昌宏、濵田勉、三浦陽子
リポソーム及びその作製方法
特願2008-110455
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Tsuruwaka Y, Konishi T, Miyawaki A, Takagi M.
Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon
Zebrafish, 4, 253-260 (2007).
doi
Tsutomu Hamada, Yoko Miura, Ken-ichi Ishii, Sumiko Araki, Kenichi Yoshikawa, Mun・fdelanji Vestergaard, and Masahiro Takagi
Dynamic Processes in Endocytic Transformation of a Raft-Exhibiting Giant Liposome
J. Phys. Chem. B, 111, 10853-10857 (2007).
doi
Movie of endocytic vesicle formation from a raft
Yoshikazu ASO, Kentaro SHIRAKI and Masahiro TAKAGI
Systematic Analysis of Aggregates from 38 Kinds of Non Disease-Related Proteins: Identifying the Intrinsic Propensity of Polypeptides to Form Amyloid Fibrils
Bioscience, Biotechnology, and Biochemistry, 71, 1313-1321 (2007).
PDF(Open Access)
M. Hase, A. Yamada, T. Hamada, D. Baigl, K. Yoshikawa
Manipulation of Cell-Sized Phospholipid-Coated Microdroplets and
Their Use as Biochemical Microreactors
Langmuir, 23, 348-352 (2007).
doi
濵田勉、高木昌宏
生体モデル膜ラフトドメインの動的構造
化学と生物, 45, 436-438 (2007).
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Takafumi Konishi, Sachiko Matsumoto, Yusuke Tsuruwaka, Kentaro Shiraki, Kazumasa Hirata, Yutaka Tamaru, and Masahiro Takagi.
Enhancing the tolerance of zebrafish (Danio rerio) to heavy metal toxicity by the expression of plant phytochelatin synthase.
J. Biotechnol. 122, 316-325 (2006). PDF(Open Access)
Matsumura H, Takahashi H, Inoue T, Yamamoto T, Hashimoto H, Nishioka M, Fujiwara S, Takagi M, Imanaka T, Kai Y
Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis Strain KOD1
Proteins: Structure, Function, and Bioinformatics, 63, 711-715 (2006).
doi
A. Yamada, T. Yamanaka, T. Hamada, M. Hase, K. Yoshikawa, D. Baigl
Spontaneous Transfer of Phospholipid-Coated Oil-in-Oil and Water-in-Oil
Micro-Droplets through an Oil/Water Interface
Langmuir, 22, 9824-9828 (2006).
doi
Masahiko Hase, Ayako Yamada, Tsutomu Hamada, Kenichi Yoshikawa
Transporting of a Cell-Sized Phospholipid Vesicle Across Water/Oil Interface
Chemical Physics Letters 426, 441-444 (2006). doi
Daisuke Saeki, Tsutomu Hamada, Kenichi Yoshikawa
Domain Growth Kinetics in a Cell-sized Liposome
Journal of the Physical Society of Japan, 75, 013602 (2006).
doi
工藤基徳、白木賢太郎、高木昌宏
小分子添加に・謔驛^ンパク質の凝集抑制
バイオサイエンスとインダストリー, 64, 323－327 (2006).
浦野泰照、高木昌宏
細胞・個体レベルでストレスをサーベイする
一細胞定量解析の最前線―ライフサーベイヤ構築に向けて―,シーエムシー出版, 94-111(2006).
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【122】Fumiyosshi Okazaki, Kentaro Shiraki, Yutaka Tamaru, Toshiyoshi Araki and Masahiro Takagi.
The first thermodynamic characterization of b-1,3-xylanase from a marine bacterium.
Protein J. 24, 413-421 (2005)
【121】Takafumi Konishi, Keitaro Kato, Toshiyoshi Araki, Kentaro Shiraki, Masahiro Takagi, and Yutaka Tamaru.
Molecular cloning and characterization of alpha-class glutathione S-transferase genes from the hepatopancreas of red sea bream, Pagrus major.
Comp Biochem Physiol. C Toxicol. Pharmacol. 140, 309-320 (2005)
【120】Motonori Kudo, Kentaro Shiraki, and Masahiro Takagi.
Stretched-exponential analysis of heat-induced aggregation of apo-concanavalin A.
Protein J. 24, 193-199 (2005).
【119】Shingo Nishikori, Kentaro Shiraki, Shinsuke Fujiwara, Tadayuki Imanaka, and Masahiro Takagi.
Unfolding mechanism of a hyperthermophilic protein O6-methylguanine-DNA methyltransferase.
Biophysical Chemistry 116, 97-104 (2005)
【118】Duanpen Sandee, Sumalee Tungpradabku, Yoichi Kurokawa, Kiichi Fukui1, and Masahiro Takagi.
Combination of Dsb coexpression and an addition of sorbitol markedly enhanced soluble expression of single-chain Fv in Escherichia coli.
Biotechnology and Bioengineering in press (2005)
【117】Naoki Tsuji, Shingo Nishikori, Sachiko Matsumoto, Osamu Iwabe,Kentaro Shiraki, Hitoshi Miyasaka, Masahiro Takagi, Kazumasa Hirata, and Kazuhisa Miyamoto.
Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay.
Planta, 2005 in press.
【116】Kentaro Shiraki, Motonori Kudou, Ryusuke Sakamoto, Itaru Yanagihara, and Masahiro Takagi.
Amino acid esters prevent thermal inactivation and aggregation of lysozyme.
Biotechnology Progress. 21, 640-643 (2005).
【115】Takafumi Konishi, Keitaro Kato, Toshiyoshi Araki, Kentaro Shiraki, Masahiro Takagi, and Yutaka Tamaru.
A new class of glutathione S-transferase from the hepatopancreas of the red sea bream, Pagrus major.
Biochem.J. 388, 299-307 (2005)
【114】Yukie Mitsuda, Kumi Tsuruhata, Emi Hifumi, Masahiro Takagi and Taizo Uda.
Investigation of active form of catalytic antibody light chain 41S-2-L.
Immunology Letters 96, 63-71 (2005)
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【113】Shingo Nishikori, Kentaro Shiraki, Shinsuke Fujiwara, Tadayuki Imanaka and Masahiro Takagi.
Equilibrium and kinetic stability of a hyperthermophilic protein, O6-methylguanine-DNA methyltransferase in various extreme conditions.
J. Biochem.(Tokyo) 136, 503-508 (2004)
【112】Kentaro Shiraki, Motonori Kudou, Shingo Nishikori, Harue Kitagawa, Tadayuki Imanaka and Masahiro Takagi.
Arginine ethylester prevents thermal inactivation and aggregation of lysozyme.
Eur. J.Biochem. 271,3242-7 (2004)
【111】Naoshige Izumikawa, Kentaro Shiraki, Shinsuke Fujiwara,Tadayuki Imanaka, and Masahiro Takagi.
Biophysical analysis of heat-induced structural maturation of glutamate dehydrogenase from a hyperthermophilic archaeon.
J.Biosci.Bioeng.97,305-309 (2004)
【110】Shingo Nishikori, Kentaro Shiraki, Kiyonobu Yokota, Naoshikge Izumikawa, Shinsuke Fujiwara, Hiroshi Hashimto, Tadayuki Imanaka and Masahiro Takagi.
Mutational effects on O6-methylguanine-DNA methyltransferase from hyperthermophile:contribution of ion-pair network to protein thermostability.
J. Biochem.(Tokyo)135,525-532 (2004)
【109】Naoki Tsuji, Shingo Nishikori, Osamu Iwabe, Kentaro Shiraki,Hitoshi Miyasaka, Masahiro Takagi, Kazumasa Hirata, and Kazuhisa Miyamoto
Characterization of phytochelatin synthase-like protein encoded by alr0975 from a prokaryote, Nostoc sp. PCC 7120.
Biochem.Biophys.Res.Com. 315,751-5.(2004)
【108】Sachiko Matsumoto, Kentaro Shiraki, Naoki Tsuji, Kazumasa Hirata, Kazuhisa Miyamoto and Masahiro Takagi.
Functional analysis of phytochelatin synthase from Arabidopsis thaliana and its expression in Escherichia coli and Saccharomyces cerevisiae.
Science and Technology of Advanced Materials (STAM), 5, 377-381 (2004).
【107】Motonori Kudou, Kentaro Shiraki and Masahiro Takagi.
Characterization of heat-induced aggregates of concanavalin a using fluorescent probes.
Science and Technology of Advanced Materials (STAM), 5, 339-341 (2004).
【106】Kentaro Shiraki, Shingo Nishikori, Shinsuke Fujiwara, Tadayuki Imanaka and Masahiro Takagi.
Contribution of protein-surface ion pairs of a hyperthermophilic protein on thermal stability in high ionic strength condition.
J.Biosci.Bioeng., 97, 75-77 (2004)
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【105】Motonori Kudou, Kentaro Shiraki, Shinsuke Fujiwara, Tadayuki Imanaka and Masahiro Takagi.
Prevention of thermal inactivation and aggregation of lysozyme by polyamines.
Eur.J.Biochem., 270, 4547-4554 (2003)
【104】Kentaro Shiraki, Masao Tsuji, Yoshiteru Hashimoto, Kenzo Fujimoto, Shinsuke Fujiwara, Masahiro Takagi and Tadayuki Imanaka.
Genetic, enzymatic, and structural analyses of phenylalanyl-tRNA synthetase from Thermococcus kodakaraensis KOD1.
J. Biochemistry (Tokyo), 134,567-574 (2003)
【103】Hiroki Higashibata, Masood Siddiqui, Masahiro Takagi, Tadayuki Imanaka and Shinsuke Fujiwara
Surface histidine residue of archaeal histone affects DNA compaction and thermostability.
FEMS Microbiology Letters, 224, 17-22 (2003)
【102】Ruthadporn Sriprang, Makoto Hayashi, Hisayo Ono, Masahiro Takagi, Kazumasa Hirata, and Yoshikatsu Murooka.
Enhanced accumulation of Cd2+ by a Mesorhizobium sp.transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase.
Appl.Eiviron.Microbiol. 69:1791-1796.(2003)
【101】Kentaro Shiraki, Motonori Kudou, Yoshinobu Aso and Masahiro Takagi.
Dissolution of protein aggregation by small amine compounds"
Science and Technology of Advanced Materials (STAM), 4, 55-59(2003).
【100】Naoki Tsuji, Nayumi Hirayanagi, Osamu Iwabe, Takashi Namba,Mariko Tagawa,Shiho Miyamoto, Hitoshi Miyasaka, Masahiro Takagi, Kazumasa Hirata, Kazuhisa Miyamoto.
Regulation of phytochelatin synthesis by zinc and cadmium in marine green alga, Dunaliella tertiolecta.
Phytochemistry 62(3):453-459 (2003).
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【99】Yoshihisa Hagihara, Kentaro Shiraki, Tsutomu Nakamura, Koichi Uegaki, Masahiro Takagi, Tadayuki Imanaka and Noboru Yumoto.
Screening for stable mutants with amino-acid pairs substituted for the disulfide bond between residues 14 and 38 of bovine pancreatic trypsin inhibitor (BPTI).
J. Biol. Chem. 277,51043－51048 (2002)
【98】Duanpen Sandee, Sumalee Tungpradabkul, Manae Tsukio, Tadayuki Imanaka, Masahiro Takagi.
Construction and high cytoplasmic expression of a tumoricidal single-chain antibody against hepatocellular carcinoma.
BMC Biotechnol. Sep 12;2(1):16 (2002).
【97】Kentaro Shiraki, Motonori Kudou, Shinsuke Fujiwara, Tadayuki Imanaka and Masahiro Takagi.
Biophysical effect of amino acids on the prevention of protein aggregation.
J. Biochemistry (Tokyo)132,591-595. (2002)
【96】Sung-Jong Jeon,Shinsuke Fujiwara, Masahiro Takagi,Takeshi Tanaka and Tadayuki Imanaka.
Tk-PTP, protein tyrosine/serine phosphatase from hyperthermophilic archaeon Thermococcus kodakaraensis KOD1:Enzymatic characteristics and identification of its substrate proteins.
Biochem. Biophys. Res. Commun. 295,508-514(2002)
【94】Masahiro Takagi, Hiroyuki Satofuka, Satoshi Amano, Haruo Mizuno, Yutaka Eguchi, Kazumasa Hirata, Kazuhisa Miyamoto,Kiichi Fukui and Tadayuki Imanaka.
Cellular toxicity by cadmium ion and its detoxification by heavy metal specific plant peptides, phytochelatins expressed in mammalian cells.
J.Biochemistry(Tokyo) 131, 233-239 (2002)
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【93】Yoshiteru Hashimoto, Tomoko Yamamoto, Shinsuke Fujiwara,Masahiro Takagi and Tadayuki Imanaka.
Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001
J. Bacteriol., 183, 5050-5057 (2001)
【92】Tadayuki Imanaka and Masahiro Takagi.
Evolution of PCR Enzymes: Towards a better PCR system based on a KOD DNA polymerase.
J. Chin. Inst. Chem. Engrs., 32, 277-288 (2001)
【91】Masahiro Takagi, Yasushi Kai and Tadayuki Imanaka
Methylguanine methyltransferase from Thermococcuskodakaraensis KOD1.
Methods Enzymol.334, 239-248 (2001)
【90】Shinsuke Fujiwara, Masahiro Takagi and Tadayuki Imanaka.
Chaperonin from Thermococcus kodakaraensis KOD1.
Methods Enzymol.334, 293-301 (2001)
【89】Kentaro Shiraki, Shinsuke Fujiwara, Tadayuki Imanaka,and Masahiro Takagi.
Conformational stability of a hyperthermophilic protein in various conditions for denaturation.
Electrochemistry.69, 949-952 (2001)
【88】Hiroyuki Satofuka, Toshiaki Fukui, Masahiro Takagi, Haruyuki Atomi and Tadayuki Imanaka
Metal-binding properties of phytochelatin-related peptides.
J.Inorg.Biochem. 86,595-602(2001)
【87】Kentaro Shiraki, Shingo Nishikori, Shinsuke Fujiwara,Hiroshi Hashimoto, Yasushi Kai, Masahiro Takagi, and Tadayuki Imanaka.
Comparative analyses of the conformational stability between a hyperthermophilic protein and its mesophilic counterpart.
Eur.J.Biochem. 268, 4144-4150(2001)
【86】Yuji Kannan,Yuichi Koga, Yohei Inoue, Mitsuru Haruki, Masahiro Takagi, Tadayuki Imanaka, Masaaki Morikawa, and Shigenori Kanaya.
Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence.
Appl. Environ. Microbiol. 67,2445-52 (2001)
【85】Hiroshi Hashimoto, Motomu Nishioka, Shinsuke Fujiwara, Masahiro Takagi, Tadayuki Imanaka, Tsuyoshi Inoue, Yasushi Kai.
Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1.
J Mol Biol.306, 469-477 (2001)
【84】Motomu Nishioka, Hiroshi Mizuguchi, Shinsuke Fujiwara, Shusuke Komatsubara, Masao Kitabayashi, Hideki Uemura, Masahiro Takagi and Tadayuki Imanaka.
Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease deficient mutant enzyme.
J. Biotechnol. 88,141-149 (2001)
【83】Michi Izumi, Shinsuke Fujiwara, Kentaro Shiraki, Masahiro Takagi, Kiichi Fukui, and Tadayuki Imanaka.
Utilization of immobilized archaeal chaperonin for enzyme stabilization.
J. Biosci. Bioeng. 91, 316-318 (2001)
【82】Hiroshi Mizuguchi, Haruo Mizuno, Kiyoshi Yasukawa, Takahiko Ishiguro, Kiichi Fukui, Tadayuki Imanaka, and Masahiro Takagi
Enhanced signal transduction by a directly fused protein of interleukin-6 and its receptor.
J. Biosci. Bioeng. 91, 299-304 (2001)
【81】Michi Izumi, Shinsuke Fujiwara, Masahiro Takagi, Kiichi Fukui,and Tadayuki Imanaka.
Two kinds of archaeal chaperonin with different temperature dependency from a hyperthermophile.
Biochem. Biophys. Res. Commun. 280, 581-587 (2001).
【80】Sung-Jong Jeon,Shinsuke Fujiwara, Masahiro Takagi, Kiichi Fukui and Tadayuki Imanaka.
Unique nucleoid structure during cell division of Thermococcus kodakaraensis KOD1.
J. Biosci. Bioeng. 91, 40-43 (2001)
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