Title:
Involvement of oxysterols in membrane interaction and vesicular transport of Amyloid beta
Speaker(s):
Neha Sharma1, Keang OK Beak1, H.T.T.Phan2, Naofumi Shimokawa1, Masahiro Takagi1
1 Japan Advanced Institute of Science and Technology, Japan
2 Hanoi National University of Education, Vietnam
Abstract:
Misfolding and aggregation of Amyloid beta is the pathology in the Alzheimer's disease. Misfolded proteins causes stress to endoplasmic reticulum (ER stress), even if they are externally added. We propose that this is due to the endocytic vesicular transport. We are studying with the Jurkat cells, 7-ketocholesterol (7-KC) and 25-hydroxycholesterol (25-OH). We observed strong surface interaction in presence of 7-KC and endocytic movement in that of 25-OH. Cell viability was also affected. Intracellular calcium level in 25-OH added cells was observed higher as compare with the control. These finding will be helpful to further understanding about part of oxysterols in ER stress of neurodegenerative diseases.